Traffic Scotland are advising drivers that the A77 from Maybole to Kirkoswald is now likely to remain closed overnight.
This was due to an accident which occurred last night around 11.30pm when a lorry overturned causing a major fuel spillage on the road.
The lorry driver was taken to Ayr Hospital but was not thought to be seriously injured according to a police spokeswoman.
Earlier Traffic Scotland had thought the road would have been closed until lunchtime but these delays have now been extended.
Diversions are in place via the B7023 road.