Both the A77 and the A714 south of Girvan and the B7044 road to Ballantrae have been closed due to flooding.
Traffic Scotland say the expected duration of the closure is unknown at present.
Diversions are also in place on the A77 near Maybole due to unsafe trees.
Parts of Girvan have also flooded as water levels continue to rise following Storm Frank.
People in the village of Straiton in South Ayrshire were also being helped by the fire service to leave their properties.