The A77 between Girvan and Maybole will be closed for roadworks from midnight on Friday, March 6 until 6am on Monday morning.
Work is due to start on carriageway improvements at Dalquat, between Girvan and Maybole.
Gordon Wilson, contract director for Scotland TranServ said: “To ensure minimal disruption work has been programmed over one weekend when traffic flows are lighter however I would urge motorists to leave plenty of time for their journeys.”
A diversion will be in place throughout the works redirecting northbound traffic on the A719 from Turnberry to Pennyglen then on to the B7023 to Maybole. Southbound traffic will travel on the B7023 from Maybole, Crosshill then on to the B741 to Turnberry.
Advanced warning signs will also be in place on the approach to the works to inform motorists that roadworks are taking place.